Millipore Technical Publications |
 | DS- DS0010EN00 | |
DNA Nucleoside Controlled Pore Glass (CPG ) MediaSolid support for DNA synthesis | ||
| Lit No: | DS0010EN00 | |
| Year: | 2006 | |
| The ability for research laboratories to cost-effectively synthesize DNA and RNA molecules in-house has greatly improved over the course of the past two decades. Automated oligonucleotide synthesizers are now available in most laboratories. Readily accessible technology has allowed synthetic molecules to be pervasively used in sequencing and in polymerase chain reaction (PCR) amplification techniques. Phosphoramidite chemistry is generally used for the synthesis of the oligonucleotides, which is carried out by sequentially adding the desired nucleotide units to an immobilized oligonucleotide chain. Millipore offers DNA Nucleoside CPG (DNA-CPG) products for the solidphase synthesis of oligonucleotides using the phosphoramidite chemistry. These products consist of the DNA nucleosides, deoxyadenosine (dA), deoxycytidine (dC), deoxyguanosine (dG) and deoxythymidine (dT), covalently attached to a CPG particle (a porous borosilicate material) ready for synthesis applications. The unique characteristics of Millipore’s CPG matrix provide users with several advantages, including high yields in the synthesis of pure oligomers. A laboratory’s increased capacity improves the development process of therapeutic drugs as well as diagnostic probes on DNA chips. Click the PDF above for the full document. | |
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