Controlled Pore Glass (CPG®) Media | Data Sheet - DS1007EN00 | |
Inorganic support for liquid chromatography and related applications |
| • Rigid glass structure for a durable, incompressible and inert media • Narrow pore size distribution for efficient purification • Large internal surface area for high binding capacity |
Produced from borosilicate glass, Millipore’s CPG medium is an inorganic support for use in liquid chromatography and associated processes. The CPG matrix is effective in the chromatographic separation of a variety of biological molecules, including proteins, enzymes, carbohydrates and nucleic acids. In addition, the chemical and biochemical industries use the solid phase for the separation of viruses, cellular components and other large particles as well as for enzyme immobilization. Available in a broad range of pore diameters, Millipore’s CPG product can be customized for the size of the molecule to be purified as well as the ligand to be used.
Characteristics
In comparison to many organic solid supports, CPG media are rigid, nonabsorptive matrices unaffected by changes in a solvent system or pressure. Both organic and aqueous solvents can be used without altering pore sizes. This chromatography support is impervious to degradation caused by solutions, such as guanidine, corrosive solvents and concentrated acids. In addition, due to a lack of compaction, chromatography columns packed with CPG material can be operated at high flow rates, which are linear with pressure.
In addition to packed columns, CPG matrices can be used in fluidized (expanded) bed and stirred tank modes. In fluidized bed operations, the media may be used with a feedstock containing particulate matter to eliminate pre-filtration.
Pore Size
CPG particles are manufactured from a borosilicate base material that is heated to separate the borates and the silicates. The borates are leached from the material to create a silica glass with uniform and controlled pore sizes. This proprietary process creates a very narrow pore size distribution with at least 80% of the pores within ±10% of the mean pore diameter. In chromatography applications, a narrow pore size distribution assures clean separations with high resolution and excellent reproducibility.
Particle Size
CPG media are available in a range of particle sizes with a narrow particle size distribution. At least 80% of the particles, by weight, are within the specified mesh range. Resolution is minimally affected by particle size, however smaller particle size can present greater flow resistance and higher back pressure.
Recommended Handling
CPG matrices can be cleaned with hot concentrated nitric acid solution or regenerated by heating up to 600oC for at least 4 hours in a porcelain or Pyrex® dish. Most contaminants of a biological origin can be removed by flushing the CPG particles with concentrated solutions of sodium chloride or 1% sodium dodecyl sulfate. A mixture of chaotropic substances such as potassium thiocyanate, lithium bromide or isopropyl alcohol may be used. Due to the inherent solubility of glass, Millipore recommends an operating pH range of 1 to 9. For specific considerations, please contact Millipore’s technical service team.
Manufacturing Standards and Quality Assurance
Millipore recognizes the importance of providing regulatory support and meeting industry quality standards. All CPG products are manufactured in a dedicated facility certified to internationally recognized standards, BS EN ISO9001. The facility is subject to routine independent surveillance audit.
A CPG particle’s internal surface area relationship to pore diameter