 | Although embryonic stem (ES) cells offer enormous potential, their use is often hampered by the lack of control inherent in traditional culture methods. These largely rely on the use of serum in the media and the presence of a layer of feeder cells. Serum is a heterogeneous, undefined product that exhibits batch-to-batch variability. In addition, feeders are cumbersome to use and add even more variation. A culture system free of both serum and feeders would make experiments more reproducible and offer scientists the control necessary for in vitro differentiation studies and large scale screening projects.
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