Bløk Noise-Cancelling Reagents

Millipore Technical Publications

		DS1073EN00.pdf DS- DS1073EN00
Bløk Noise-Cancelling Reagents Protein-free blocking reagents for Western blotting
Lit No:	DS1073EN00
Year:	2010

	In Western blotting, blocking of unbound membrane sites is necessary to prevent non-specific binding of the antibodies which otherwise would lead to a high background on the blot. The traditional milk blocker can work well; however, inadvertent variations in its composition can lead to irreproducible results. Such variations include the milk’s concentration, fat content, solubility, detergent quality, and numerous other factors. Bløk reagents are a family of protein-free, noisecancelling reagents that reduce background for consistent, quality results. They are available in three room temperature-stable, ready-to-use formulations for: Chemiluminescence and chromogenic detection Fluorescence detection Phosphoprotein immunodetection The protein-free nature of Bløk reagents allows for membranes to be stained with chromogenic reagents, such as Ponceau S or Coomassie™ blue, after blocking or immunodetection. Bløk reagents have been tested and validated for Western blot, dot blot, and ELISA applications. Click the PDF above for the full document.