| Hybridoma screening is a complex process involving determination of antibody
specificity, IgG quantification, antibody isotype, antibody affinity and antibody
function in the selection of the best clones. Traditionally, these assays are performed
using different experimental techniques and multiple instrument platforms, some
of which involve long procedures. The Guava EasyCyte™ bench-top cytometer is a
96-well platform which can be utilized to screen both cells and beads and can be an
ideal space-, money-, and labor-saving solution for hybridoma researchers. In this
collaborative study, mice were immunized with human CD80 and <30 microliters of
total supernatant from each resulting hybridoma was subjected to multiple analyses
on the EasyCyte platform. The best clones were identified based on multiple criteria.
The antigen specificity was obtained using a cell-based Guava Express™ assay, quantitative
levels of IgG in supernatant were determined using the Guava RapidQuant™
Mouse IgG assay, and isotype and relative affinity were also ascertained. Using
these analyses, 6 top ranking clones from 455 tested clones were identified based
on specificity and productivity data. The Guava EasyCyte platform and assays thus
empower researchers by providing multiple layers of information in the ranking and
selection of the optimal clones. |
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