Millipore Technical Publications |
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Reducing Variation in Elispot assays | ||
| Lit No: | MK1010 | |
| Year: | 2004 | |
| The ELISPOT assay for the evaluation of T cell response to vaccine candidates requires that the number of cells in the sample be accurately determined. This application note describes a study demonstrating the use of the Guava ViaCount cell counting technique provided significantly more precise cell counts over multiple samples than manual procedures such as Trypan Blue staining or the use of a fluorescent substrate. The accuracy of the Guava ViaCount procedure was found to be equivalent to the other techniques. An additional benefit of the Guava ViaCount technology is that an electronic record is generated that is easily transferred to other databases. |
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