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Millipore Technical Publications |
 | |
Reducing Variation in Elispot assays |
| Lit No: | MK1010 |
| Year: | 2004 | |
| The ELISPOT assay for the evaluation of T cell response
to vaccine candidates requires that the number of cells
in the sample be accurately determined. This application
note describes a study demonstrating the use of the
Guava ViaCount cell counting technique provided significantly
more precise cell counts over multiple samples
than manual procedures such as Trypan Blue staining or
the use of a fluorescent substrate. The accuracy of the
Guava ViaCount procedure was found to be equivalent
to the other techniques. An additional benefit of the
Guava ViaCount technology is that an electronic record
is generated that is easily transferred to other databases. |
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| Click the PDF above for the full document. |
