| Viability assays are crucial tools in the development of
many drugs, particularly of cytotoxic agents. To best
characterize potential compounds, viability assays
must ideally be able to reproducibly and accurately
report small percentage changes in viability, provide
statistically significant quantification of biological
activity, and account for changes in cell number
during the assay. An analysis of cell number interference
on MTT, alamarBlue™, and Guava ViaCount®
viability assays was performed in the development of
a quantitative CD40 ligation assay. MTT and alamar-
Blue assays both showed significantly large statistical
variations in viability measurements due to changes in
cell numbers. Guava ViaCount’s ease of use and high
reproducibility provide significant advantages over
current viability or cytotoxicity assays where changing
cell numbers can be problematic. |
