Millipore Technical Publications |
 | Data Sheet - PF1086EN00 | |
CellCiphr™ Cytotoxicity Assay Human HepG2 Cells Cellular Systems Biology (CSB)™ KitUse the Power of Multi-Analyte Detection to Rapidly Determine Cytotoxicity | ||
| Lit No: | PF1086EN00 | |
| Year: | 2007 | |
Hepatotoxicity is a significant factor in the high failure rate of drug development, and in withdrawal of drugs from the market. Prediction of human hepatotoxicity using traditional in vitro studies has been unreliable due to poor assay and/ or reagent specificity, insufficient numbers of endpoints, and an inability to detect early stages of cytotoxicity. The Cellular Systems Biology™ approach to cytotoxicity profiling (developed by Cellumen Inc.) takes advantage of the sensitivity and multiplexing capability of high content screening (HCS) readers. HCS is an effective tool for determination of drug-induced cytotoxicity using the human hepatocellular carcinoma cell line HepG2.
The CellCiphr Cytotoxicity Assay for human HepG2 cells is an assay co-developed by Cellumen and Millipore that comprises high-quality, validated, multiplexed detection reagents and protocols for profiling eleven human cytotoxicity parameters. Cellumen’s proprietary panel, composed of unique antibody pairs and detection reagents from Millipore, allows detection and analysis of the cellular systemic response to toxin challenge. The CellCiphr Cytotoxicity Assay screens compounds for a broad range of potentially toxic effects early in the drug discovery process.