Millipore Technical Publications |
 | Data Sheet -PF1140EN00 | |
33mm Millex Filter Units for Sample PreparationWith low protein-binding Durapore membrane | ||
| Citation: | PF1140EN00 | |
| Date: | 2004 | |
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| Abstract: | Non-sterile Millex filters with Durapore membrane are ideal for preparing 10 – 100 mL of protein- containing solutions prior to chromatography or other instrument analyses. The 0.45 µm pore size (type HV) removes fine particles, which can prematurely plug chromatography columns. The 0.22 µm pore size (type GV) is recommended for sterilizing-grade filtration. Both units can be used with aqueous or mild organic solutions. | |
- Ultra-low protein binding
- Low extractables
- High operating pressure
- Low hold-up volume
- Available in 0.45 µm and new 0.22 µm
Non-sterile Millex filters with Durapore membrane are ideal for preparing 10 – 100 mL of protein- containing solutions prior to chromatography or other instrument analyses.
The 0.45 µm pore size (type HV) removes fine particles, which can prematurely plug chromatography columns. The 0.22 µm pore size (type GV) is recommended for sterilizing-grade filtration. Both units can be used with aqueous or mild organic solutions.
33 mm for Faster Flow Rate
New Millex filters are 33 mm in diameter and have almost 20% more filter surface area than our 25 mm filters. The larger filter surface area increases flow rate and throughput. It also makes it easier to filter solutionsbecause it reduces the pressure required to empty the syringe.
Low Hold-up Volume
Despite the increased membrane area, the hold-up volume (after air purge) has been reduced by 20% to 80 µL compared to 25 mm Millex filters.
Higher Operating Pressure
New Millex filters have a maximum housing pressure of 125 psig (8.5 bar), which means you can filter solutions faster than before.
Lowest Protein Binding Available
Durapore membrane is the lowest protein-binding membrane, which makes it ideal for filtration of protein-containing solutions.
Low Extractables
The materials used to manufacture this filter unit are low in extractables (refer to “Performance” section). The filter is suitable for use with aqueous samples and a variety of HPLC solvents (refer to “Chemical Compatibility”).
Automated Manufacturing Process
The Millex manufacturing process is fully automated from membrane cutting through device assembly and packaging to minimize the risk of contamination. Each membrane is inspected for defects using a digital vision system prior to assembly into the device.
Validation Guide Available
To download, visit
millipore.com/millex
Chemical Compatibility
33 mm Millex filters with PVDF membrane are suitable for use with aqueous or mild organic samples. They exhibit broad chemical compatibility, including the HPLC solvents listed below. For complete chemical compatibility information, go to www.millipore.com to view the user guide (search on document “PR01559”)
Solvent
Acetic acid
Ammonium hydroxide, 1N
Benzene
Carbon tetrachloride
Chloroform
Ethanol
Ethyl ether
Hexane
Hydrogen peroxide, 30%
Isopropyl alcohol
Methanol
Nitric acid, 6 N
Water.
Typical Performance
Three filters with PVDF membrane were tested from each manufacturer. Extracts were pooled and then analyzed under gradient mobile phase conditions using a Waters Symmetry® column (C18, 5 µm, 3.9 mm x 150 mm) on a gradient HPLC system. Baselines were offset to facilitate visual analysis. Data show that extractables from 33 mm Millex filters are comparable to 25 mm Millex filters and to other brands.