Millipore Technical Publications |
 | DS- PF174 | |
Micropure-EZ Enzyme RemoversRemove restriction and other enzymes from DNA in 30 seconds | ||
| Lit No: | PF174 | |
| Year: | 1998 | |
| Micropure-EZ provides an easy, rapid means of removing restriction and other enzymes from double-stranded (ds) DNA. The membrane in the device has a high affinity for protein but not for dsDNA. In a single 30-second spin, enzymes from the reaction mix are selectively adsorbed and DNA is recovered in a vial- undiluted and enzyme-free. If concentration or desalting of the sample is required, Microcon¨ centrifugal filter devices may be substituted for the filtrate vial, providing for DNA purification, concentration and desalting in a single unit. Unlike precipitation or "bind and elute" purification, Micropure-EZ needs little hands-on time. This is especially useful when processing multiple samples. Also, DNA recovery is not affected by the binding capacity of an adsorption matrix. Micropure-EZ will deliver minimum 85% recovery of dsDNA, higher with a quick 5-10 µL rinse. Click the PDF above for the full document. | |
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