Millipore Technical Publications |
 | PO- PS0018ENUS | |
Improving Reliability of Filter-Based Luminex xMAP Multiplex Assays Through Filter Plate and Membrane Material | ||
| Lit No: | PS0018ENUS | |
| Year: | 2006 | |
| Luminex xMAP Multiplex technology, utilizing microspheres as a solid support, continues to grow in its application for immunological research and diagnostics. This technology is frequently used, for instance, in serological assays to assess the presence of autoimmune disease. Optimization of factors such as bead recovery and non-specific reactivity can each have a significant impact on the reliability of these assays by ensuring statistically relevant analysis and preventing false positive results, respectively. We demonstrate the impact of filter plate and membrane materials on bead recovery, assay signal, and data reliability. In addition, we successfully show reduced false positive frequency in a known, clean, yet atypical serum sample subjected to concurrent detection for specific antigens. Initial findings support the role of surface interactions in the reduction of nonspecific reactivity. Thus, filter plate and membrane materials have a significant impact on the prevalence of false-positives when used with the described technology for serological assays. Click the PDF above for the full document. | |
| |
| |
