Millipore Technical Publications |
 | PO- ps1040en00 | |
Poster: Methods For The Detection of Oxidative Stress | ||
| Lit No: | ps1040en00 | |
| Year: | 2008 | |
| Oxidative stress is a cellular disorder caused by an excess of free radicals
which can occur because of increased oxidant levels, decreased anti-oxidant
amounts, or failure to repair oxidative damage induced by reactive oxygen
species (ROS). This condition has been linked to the pathogenesis of a
number of disease states including atherosclerosis, ischemia, cancer,
rheumatoid arthritis, and neurodegeneration. ROS are free radicals, reactive
molecules containing oxygen, and include superoxides, hydroxyl radicals,
hydrogen peroxides, nitric oxides, peroxynitrites, and singlet oxygens. ROS
molecules are capable of chemically modifying proteins, lipids, and nucleic
acids which potentially can alter or impair their natural biological function.
Multiple approaches for detection of modifications are needed due to the
variety of ROS molecules that exist as well as the variety of alterations they
can impart. The most common approach is to detect a modification or protein
involved in processing using an antibody. This is typically done by western
blot analysis or IHC. Approaches to detect modifications to DNA (anti-8
hydroxydeoxyguanosine or anti-8-oxoguanine), lipids (anti-neuroketal, anti-4-
hydroxynonenal), or proteins (anti-SOD1, anti-ntirotyrosine, anti-nNOS, anti-
HIF1a, and more) are commonly utilized. Another method frequently
employed to detect ROS modifications is to chemically attach a molecule to a
particular group and subsequently detect this extension. Utilizing this
methodology we illustrate detection and quantification of carbonyl
modifications using the OxyBLOTTM and OxyELISATM assays. Results for
each of these methodologies are given as well as discussions about
advantages and disadvantages of each. This will allow researchers to decide
how best to detect ROS-induced modifications based on their particular
needs ranging in scope from molecular to single cell to system wide.
Click the PDF above for the full poster. | |
| |
|
