| Presented herein is an automation compatible, high throughput method for the determination of drug concentrations in serum or plasma samples. The MultiScreen® Deep Well Solvinert filter plate permits inplate mixing and incubation of acetonitrile and serum to completely precipitate serum proteins. Filtration through the 0.45 µm PTFE membrane yields a protein-free solution with complete sample recovery for seamless integration with LC-MS/MS detection. Recoveries of three drugs (warfarin, testosterone and propranolol) from bovine serum are shown to be reproducible by LC-MS/MS over a broad concentration range with the deep well filter plate using Tecan Genesis®, Hamilton MICROLAB® STAR and Beckman Multimek™ automation systems. Correlation of automated methods to manual and centrifugal methods is presented and illustrates that sample preparation for LC-MS/MS analysis using a deep well filter plate is a fast and effective alternative to centrifugation. |
