Millipore Technical Publications |
 | PO- ps1057en00 | |
Poster: Improved Method For Adeno and Lenti- Virus Purification and Titration | ||
| Lit No: | ps1057en00 | |
| Year: | 2008 | |
| Highly purified virus is a requirement for many downstream applications as cellular debris and proteins derived from culture media in crude virus preparation can be toxic to target cells or can cause immunogenic reactions when used for in vivo injection. Conventional virus purification methods based on density gradient ultracentrifugation (most widely used sucrose or cesium) require technical expertise and are time-consuming. These methods often result in low virus recovery. We report here a fast and easy method for Adenovirus and Lentivirus purification. In our purification method, viral particles are captured on chemically modified membranes. Our specific wash and elution protocol allows for a quick and efficient purification resulting in high yields of pure virus and doesn’t affect infectivity. In addition, we developed an accelerated method for virus titration and detection. Standard plaque assay for determining infectious titers is tedious, and takes one or weeks. Our method is ELISA based, can be completed within two hours, and produces consistent results. The method can be used to characterize crude virus preparations from cell lysates as well as purified virus. Click the PDF for the entire poster. | |
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