Millipore Technical Publications |
 | PO- PS1093EN00 | |
EC50 of reagents used in blocking heterophilic interference in immunoassays | ||
| Lit No: | PS1093EN00 | |
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AbstractObjective: To demonstrate a quantitative model for evaluating the effectiveness of commercially available blocking reagents in blocking heterophilic interference in immunoassays.Relevance: Heterophile antibodies are well known interferences that affect many immunoassay formats. Among the most commonly encountered Heterophile antibodies are human anti mouse antibodies (HAMA) and rheumatoid factor (RF), a type of heterophilic antibody with non-specific affinity for the Fc fragment of IgG. To avoid false positive or falsely elevated results immunoassay manufacturers typically add commercially prepared blocking reagents to prevent interference from heterophilic antibodies. Unfortunately quantitative data on the performance of various blocking agents is scarce. We present quantitative performance data on reagents commercially used in blocking heterophilic antibody interference.
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