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Millipore Technical Publications

ps1226en00.pdf
PO- ps1226en00

Poster: ECM Cell Culture Optmization

Lit No:ps1226en00
Year:2008


Extracellular matrix (ECM) proteins are produced intracellularly and are subsequently secreted into the surrounding cellular medium, actively regulating a diverse range of cell functions including adhesion, differentiation, proliferation, migration, invasion and survival. ECM proteins are critical for in vitro culture of many known cell types including neural stem cells and are key building blocks of the physiologically normal 3-D cellular environment. A primary utility of ECMs in in vitro cell culture is to promote cellular adhesion while maintaining cell viability and maximizing cell proliferation for downstream cell-based applications. In cases where optimal cell growth conditions are not well defined and critical adhesion protein requirements are unknown, identifying the ideal ECM protein(s) at functionally relevant concentration(s) is a time-consuming and laborintensive process.

Here, we present a multiparametric assay that allows researchers to:
  • 1. quickly identify ECM(s) that best promote cell-type specific adhesion and
  • 2. pinpoint the optimal ECM concentration required to promote maximum adhesion for a particular cell type.

Click the PDF above for the full document.