Millipore Technical Publications |
 | PO- PS2148EN00 | |
Development and Validation of Automated Electrophysiology Assays for Voltage-gated Sodium Channels | ||
| Lit No: | PS2148EN00 | |
| Year: | 2008 | |
| Millipore has developed a panel of robust electrophysiology-based assays for profiling compounds against each of the Nav
subtypes, from 1.1 to 1.8, stably expressed in mammalian cell lines. These assays use two different automated platforms
(IonWorks® and PatchXpress™ systems) which, like manual patch clamp, offer relatively accurate voltage clamp and the ability to
directly record ionic current through the channel. Since the principles underpinning these technologies are so similar to manual
patch clamp, the high false positive/negative rates typically associated with other screening methods are avoided and, crucially,
they can be used to monitor use- and voltage-dependence.
Here we demonstrate how the improved robustness and reproducibility of population patch clamp (PPC) available with the IonWorks Quattro™ instrument can be exploited to reliably screen relatively large numbers of compounds against Nav channels. Click the PDF above for the full document.
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