Millipore Technical Publications |
CellCiphr – Cellular Systems Biology Assay for Human Hepatotoxicity | Product Catalogue | |
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| Hepatotoxicity is a major factor in both the high fail rate of drug development and withdrawal of drugs from the market. Millipore and Cellumen have co-developed the CellCiphr cytotoxicity profiling assay, a Cellular Systems Biology kit comprising detection reagents/protocols for profiling eleven hepatotoxicity endpoints in human HepG2 cells: Cell Loss, Cell Cycle Arrest, DNA Degradation/Apoptosis, Nuclear Size, Oxidative Stress, Stress Kinase Activation, DNA Damage, Mitochondrial Membrane Potential, Mitochondrial Mass, Mitotic Arrest and Cytoskeletal Integrity. The CellCiphr Cytotoxicity Kit allows analysis of all parameters at 3 time-points for 4 control toxins and up to 16 unknown compounds in two 10-point dose response curves. Reagents and protocols have been validated for analysis on both the IN Cell Analyzer 1000 and ArrayScan high-content screening systems. Our data demonstrate the kit’s effectiveness in the detection of each parameter’s response to control toxins. The combination of unique antibody pairs and detection reagents allows comprehensive assessment and analysis of the cellular systemic response to toxin challenge. Click PDF above to view entire poster. |