 | EMD Millipore has developed a novel bioreactor monitoring approach, Mobius SensorReady technology, which is used to monitor and control its Mobius CellReady 200 L single-use bioreactor system. The Mobius SensorReady technology is an external circuit containing sampling ports and a configurable number of additional probe port options sterilely connected to the CellReady bioreactor at the time of use. The Mobius SensorReady options include redundant pH and dissolved oxygen (DO) probes, optical single-use sensor technology and supplementary probe ports as desired. This modular design provides the user with ultimate flexibility to monitor and control their bioreactor processes with a variety of both conventional and cutting edge sensor technologies. Mobius SensorReady technology also contains a “bearingless” pump with a low shear rate, which is based on magnetic levitation, and a non-invasive flow measurement device that ensures a continuous and efficient flow of well-mixed bioreactor contents to the sensors. Here we present data from several studies conducted to demonstrate the utility of the Mobius SensorReady technology for real time measurements used to monitor and control bioreactor conditions. Concerns around the potential impact of the pump on mammalian cell viability through the introduction of shear stress were mitigated through in-depth cell culture studies. Additionally, we investigated the potential effects of shear stress on cells using a flow contraction device with several cell lines. The results of these studies have demonstrated that the Mobius SensorReady technology can be used effectively to monitor bioreactor operation with negligible impact on cell viability. |
