Millipore Technical Publications |
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Utility of The Mobius® CellReady 3 L Single-Use BioreactorFor Upstream Process Development Applications | ||
| Lit No: | PS32340000 | |
| Year: | 2011 | |
| Abstract Single-use processing solutions have become an important trend in the biopharmaceutical industry spanning both upstream and downstream applications. The advantages of the single-use approach to industrial cell culture versus traditional stainless steel and/or glass bioreactors has resulted in the recent commercialization of several single-use bioreactors (SUBs) at small (3 L-15 L), intermediate (50-500 L) and large scales (>1000 L). EMD Millipore has recently introduced the Mobius CellReady 3 L bioreactor, a SUB that shares the features of conventional bioreactors but offering ease-of-use benefits associated with single-use technology that was developed for the optimization of mammalian cell growth and expression. Detailed characterization of this vessel, both in terms of function for CHO cell growth and expression and volumetric mass transfer coefficients (kLa) for oxygen, as compared to glass bioreactors, was performed. The results demonstrate similar cell culture performance and kLa values. Based on these results, we have been able to streamline our cell culture work by using the 3 L CellReady bioreactors to scale up inoculum (i.e., seed-train) for large scale bioreactor experiments. By pooling the cultures from 2-3 small scale single-use bioreactors, sufficient biomass was readily achieved to inoculate directly into the 200 L CellReady Bioreactor. There, a 40 L n-1 step preceded the 200 L production experiments. Subsequently, the 3 L CellReady bioreactors were used to monitor performance of the 200 L large-scale cell culture experiments as satellite bioreactors. Tracking of cell culture performance in this fashion was both effective and convenient. In addition to its use in process characterization and development work, this study demonstrated that the small size and portability of the 3 L CellReady bioreactor make it an ideal platform for both seed-train and satellite applications. Click the PDF above for the full document. | |
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