Millipore Technical Publications |
 | PO- PS3312EN00 | |
Recombinant AlbuminIts role in cryopreservation and cell recovery | ||
| Lit No: | PS3312EN00 | |
| Year: | 2010 | |
| Cryopreservation is widely used to generate large stocks or cell banks that can be stably stored for short and long periods ready to be recovered and used as required in both research and industrial applications. Demand is increasing for this step to be devoid of serum or any animal-derived components, especially when it precedes an animalfree biomanufacturing process. Since one of the functions of albumin, the most abundant protein in serum, is as a carrier protein for many small molecules, it is a likely candidate to replace serum in cryopreservation. Recombinant albumin provides an animal-component-free (ACF) option without the risks and regulatory challenges associated with serum. Here, we examine the ability for two different formats of ACF albumin products, CellPrime rAlbumin AF-G and CellPrime rAlbumin AF-S to replace serum in the cryopreservation of Chinese Hamster Ovary (CHO) and Sp2/0 cells, respectively. Successful replacement of fetal bovine serum (FBS) with CellPrime recombinant human albumin, as shown in this study, allows scientists to develop defined serum-free ACF cryopreserving and recovery media. Click the PDF above for the full document. | |
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