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Millipore Technical Publications


PS4323EN00_EMD.pdfPS4323EN00_EMD.pdf
PO- PS4323EN00

Novel and Rapid Method to Assess Proliferative Properties of Mammalian Cells by Monitoring Cell Size Distributions.

Lit No:PS4323EN00
Year:2011



Introduction

We have employed the Scepter system, a handheld, automated cell counter that instantly reports cell size/volume distributions as well as a Guava® benchtop microcapillary flow cytometer, to investigate the relationship between cell cycle, cell proliferation rate, and cell size/volume. Our results show that monitoring the shape of the cell size/volume distribution is an useful method for a rapid QC assessment of your cell culture (apoptosis, cell cycle disruption and proliferation). Further in depth detailed analysis can be acquired afterwards using Guava.

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