Millipore Technical Publications |
 | TN- TB1025EN00 | |
Effect of Membrane Filter Pore Size on Microbial Recovery and Colony Morphology | ||
| Lit No: | TB1025EN00 | |
| Year: | 2001 | |
| Membrane filters with a 0.45 µm pore size have long been recognized as the standard for growth of microorganisms. However, there is little published literature comparing the effects of different pore sizes on colony size and recovery. This study takes a broad look at the influence of different pore sizes on some common microorganisms. It provides data on the effect of pore size on growth and recovery. The study compares a variety of microorganism/media combinations on a range of pore sizes: 0.22, 0.45, 0.7, 0.8, and 1.2 µm. The filtration method used in the study was a standard glass funnel and base with vacuum. Test filters were plated on solid (agar) media and compared against spread plates. |
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