Millipore Technical Publications |
 | TN- TB1026EN00 | |
Increasing Purity on ProSep-vA Affinity Chromatography Media | ||
| Lit No: | TB1026EN00 | |
| Year: | 2006 | |
| ProSep-vA protein A affinity chromatography media enables high-throughput purification for monoclonal antibodies (MAbs) together with long lifetime and broad process flexibility. ProSep-vA media, as with all chromatography media, can exhibit a degree of non-specific binding, which is typically characterized by co-elution of host cell proteins (HCP) together with the MAb. Typically the levels of non-specific binding (NSB) to ProSep-vA media are very low and the purity of the eluate can routinely exceed 96%, and the residual HCP levels can readily be cleared by subsequent purification steps. In situations where lower HCP levels are desirable, an intermediate wash step can be used. This is aimed at selecting conditions for the post load wash, which will elute HCP and other contaminants, while leaving the MAb bound. Subsequent elution of the MAb will result in a higher purity product pool. Click the PDF above for the full document. | |
| |
| |
