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Millipore Technical Publications


TB1175EN00.pdf
TN- TB1175EN00

Determination of Dynamic Binding Capacity for ProSep-vA Media

Lit No:TB1175EN00
Year:2005


The dynamic binding capacity of a chromatography media is the amount of target protein the media will bind under actual flow conditions before significant breakthrough of unbound protein occurs. As this parameter reflects the impact of mass transfer limitations that may occur as flow rate is increased, it is much more useful in predicting real process performance than a simple determination of saturated or static capacity.

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