Millipore Technical Publications |
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Muse™ MultiCaspase AssayRapid Detection of Cellular Caspase Activity | ||
| Lit No: | TB5543EN00 | |
| Year: | 2011 | |
| Assay Principle The Muse™ MultiCaspase Assay detects the activity of caspases 1, 3, 4, 5, 6, 7, 8 and 9. The assay simultaneously determines the percentage and concentration of cells with caspase activity, in combination with a dead cell dye. Included in the kit are: (1) A fluorogenic, derivatized VAD-peptide that can detect the activity of multiple caspases (2) A cell death impermeant DNA binding dye, 7-AAD that provides information on membrane integrity. The VAD-peptide is derivatized with a fluorescent group and a fluoromethylketone irreversible caspase inhibitor moiety, generating a Fluorescent-Labeled Inhibitor of Caspases (FLICA). The peptide is membranepermeable and non-cytotoxic. It binds to activated caspases with resulting fluorescent signal proportional to the number of active caspases in the cell which increases signal in the caspase activity axis. The dead cell marker, 7-AAD, is excluded from live (healthy) and caspase positive cells, but enters and stains membrane compromised later stage apoptotic and dead cells that show increased fluorescence in the viability axis. Click on the PDF above for the full document. |
