Millipore Technical Publications |
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Muse™ MitoPotential AssayEarly and Sensitive Detection of Cellular Health Perturbation | ||
| Lit No: | TB5544EN00 | |
| Year: | 2012 | |
| The Muse™ MitoPotential Assay utilizes the MitoPotential Reagent, a cationic, lipophilic dye, to detect changes the mitochondrial membrane potential and 7-AAD as an indicator of cell death. Included in the kit are: 1) MitoPotential Reagent: High membrane potential drives accumulation of MitoPotential Reagent within the inner membrane of intact mitochondria resulting in high fluorescence. Cells with depolarized mitochondria demonstrate a decrease in fluorescence. This parameter is displayed on the MitoPotential axis. (2) A dead cell marker (7-AAD) is also used as an indicator of cell membrane structural integrity and cell death. It is excluded from live, healthy cells, as well as early apoptotic cells. Dead cells thus show increased fluorescence in the Viability axis. Click the PDF above for the full document. |
