Millipore Technical Publications |
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Muse™ Caspase-3/7 AssayA Mix-and-Read Assay for Apoptosis Detection | ||
| Lit No: | TB5567EN00 | |
| Year: | 2011 | |
| Assay Principle The Muse™ Caspase-3/7 Assay enables simultaneous measurement of caspase-3/7 activity and cell death in a single assay. The assay determines the count and percentage of cells in various stages of apoptosis based on caspase 3/7 activity in combination with a dead cell dye. Included in the kit are: (1) The novel, fluorogenic Muse™ Caspase-3/7 reagent for detecting caspase-3/7 activity (2) A cell death dye, 7-AAD, that provides information on membrane integrity. The cell membrane-permeable, nontoxic Muse™ Caspase-3/7 reagent contains a DNA-binding dye that is linked to a DEVD peptide substrate. While still conjugated to DEVD, the dye is unable to bind DNA. Cleavage by active caspase-3/7 in the cell results in release of the dye, translocation to the nucleus, binding of the dye to DNA and high fluorescence. The dead cell marker, 7-AAD, is excluded from live (healthy) and early apoptotic cells, but enters membrane-compromised later stage apoptotic and dead cells. Late apoptotic and dead cells thus show increased fluorescence in the viability axis. | |
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