Millipore Technical Publications |
 | TN- TN072 | |
Sample Preparation of Peptides or Proteins Prior to Electrospray/Nanoelectrospray MSUsing ZipTipC18, ZipTipµ-C18 and ZipTipC4 Pipette Tips | ||
| Lit No: | TN072 | |
| Year: | 1999 | |
| ZipTip is a 10 µL (P-10) pipette tip with a bed of chromatography media fixed at its end such that there is no dead volume. It is intended for purifying and concentrating femtomoles to picomoles of protein, peptide or oligonucleotide samples prior to analysis, providing better data quality. The sample is aspirated and dispensed through ZipTip to bind, wash, and elute. Recovered samples are contaminant-free and eluted in 0.5-4 µL for direct transfer to a mass spectrometer target or vial. This protocol provides a guideline for using ZipTipC18 and ZipTipC4 to facilitate protein or peptide binding, salt and detergent removal, and sample elution for direct electrospray/nanoelectrospray MS analysis. C18 is offered in two bed volumes: ZipTipC 18(a standard bed of 0.6 µL for sample elution in 1 to 4 µL) and ZipTipµ-C18 (a micro bed of 0.2 µL for elution in < 1 µL). |
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