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Millipore Technical Publications


Protein Dot Blotting using Immobilon-P

Lit No:TN1004ENUS

Dot blotting is a method of applying proteins directly onto a membrane. A dissolved sample is pulled through the membrane by either applying a vacuum, absorption or intrusion; proteins bind to the membrane and the other sample components pass through. The proteins on the membrane are then available for analysis. This technique can be used either as a qualitative method for rapid screening of a large number of samples or as a quantitative technique. It is especially useful for testing the suitability of experimental design parameters.

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