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Millipore Technical Publications


AN- TP001EN00

Protein Blotting Handbook: 6th Edition

Tips and Tricks

Lit No:TP001EN00

Since its introduction in 1979 (Towbin et al., 1979), protein blotting has become a routine tool in research laboratories. It is traditionally used to detect low amounts of proteins in complex samples or to monitor protein expression and purification. The simplest protein blotting procedure, known as dot blot or slot blot, uses vacuum filtration to transfer protein onto a microporous membrane. While this method may provide qualitative information about total protein expression levels and can be performed on multiple samples in parallel, it lacks information on protein molecular weight. Also, specificity can be compromised as protein degradation products or post-translationally modified isoforms may be detected along with the intact protein.

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