Millipore Technical Publications |
 | UG-LSKMAGXX | |
PureProteome Protein A and Protein G Magnetic Beads User Guide | ||
| Lit No.: | LSKMAGXX | |
| Rev No.: | B | |
| Year: | 2010 | |
| Rev Date: | 08/01/2010 | |
| The Fc portion of a variety of immunoglobulins (Ig) is known to bind to several bacterial proteins, including Protein A from Staphylococcus aureus and Protein G, which is produced by various Streptococcus species. The binding affinities of immunoglobulins for Protein A and Protein G vary depending on both the species used to generate the antibody, as well as the antibody’s isotype (see Table 1 for details). Both Protein A and Protein G can be extremely useful research tools for applications involving antibodies. PureProteome Protein A and Protein G Magnetic Beads further enhance the utility of Protein A and Protein G by covalently coupling these reagents to paramagnetic affinity media (i.e., magnetic beads). These beads provide users with a bench-top platform for the rapid, reproducible separation of immunoglobulins from complex mixtures such as serum samples, tissue culture supernatants, and cellular lysates. To achieve separation, samples are mixed with PureProteome Protein A or Protein G Magnetic Beads for a short period of time to bind the immunoglobulins. The beads are then isolated using a magnetic stand, followed by several wash steps to remove unbound proteins. Finally, the bound proteins are eluted at high purity. This magnetic system is a convenient format for serum depletion, immunoprecipitation, or other applications that employ Protein A or Protein G. Click the PDF above for the full document. | |
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