Millipore Technical Publications

Protocol: Sodium Orthovanadate Activation

1. Prepare a 200 mM solution of sodium orthovanadate.
2. Adjust the pH to 10.0 using either 1N NaOH or 1N HCl. The starting pH of the sodium orthovanadate solution may vary with lots of the chemical. At pH 10.0 the solution will be yellow.
3. Boil the solution until it turns colorless (approximately 10 minutes).
4. Cool to room temperature.
5. Readjust the pH to 10.0 and repeat steps 3 and 4 until the solution remains colorless and the pH stabilizes at 10.0.
6. Store the activated sodium orthovanadate as aliquots at -20°C.