Millipore Technical Publications
Protocol: Sodium Orthovanadate Activation
|Sodium orthovanadate should be activated for maximal inhibition of protein phosphotyrosyl-phosphatases . |
This procedure depolymerizes the vanadate, converting it into a more potent inhibitor of protein tyrosine phosphatases. Please note that adding DTT rapidly inactivates sodium orthovanadate.
Reference: Gordon, J.: Methods Enzymol. (1991) 201, 477-482