| The Guava Check Kit is used to verify the performance of your Guava PCA,
Guava PCA-96, Guava PCA-96 AFP, Guava EasyCyte™, Guava EasyCyte Plus, or
Guava EasyCyte Mini System by assessing counting accuracy and fluorescence
detection using a standardized fluorescent bead reagent. The kit contains
Guava Check Beads and Diluent. Running the Guava Check Procedure daily,
before performing any assays, is important to assure that your Guava Systems
provide reliable, accurate results. Guava Check Beads are provided as a
concentrated fluorescent bead suspension of known concentration. To run
the Guava Check Procedure, mix the bead reagent with Guava Check Diluent
at the recommended dilution to yield a bead suspension at a specific target
concentration. The Guava Check Software Module analyzes the results from
three sequential data acquisitions of this bead suspension. The count results
are displayed on the screen for comparison with the known target value.
Particle counts that are outside the expected range are displayed in red. Count
results, forward scatter and side scatter (optional on some systems) signal
intensity, and mean fluorescence intensities are displayed. The averages and
%CV values for these results are calculated and also displayed. These results
and the instrument settings are saved to a spreadsheet file. The results from all
Guava Check Procedures are appended to a file, providing a history of data
that allows you to monitor the performance of your Guava System. |
