| The Guava ViaCount® assay provides absolute cell count and viability data on
cell suspensions from a variety of cultured mammalian cell lines. However, cell
samples containing cell aggregates may not yield accurate and consistent results
with Guava ViaCount Reagent alone. The differential staining pattern of viable
and non-viable cells in clumps are difficult to resolve and assess individually.
Cell aggregates may clog or be excluded from the flow cell, hampering the assay.
The ViaCount Cell Dispersal Reagent is an enzymatic reagent formulated to
gently disaggregate the cells in clumps, yielding a more uniform cell suspension
for assay. This assay has been optimized for Chinese Hamster Ovary (CHO-K1)
cell lines that have been adapted to suspension culture and tend to form cell
aggregates. However, Guava ViaCount CDR may be useful for disaggregating
other types of clumpy suspension cultures, yielding improved consistency of
counts in the ViaCount application. |
