| MultiScreen384SEQ plates are intended for the removal of salts and unincorporated dye terminators from sequencing reactions prior to DNA sequencing. The protocol is fast, easy to perform, and has proven effective in removing both major types of commercially available energy transfer dyes (BigDye® Terminators and DYEnamic® ET Terminators). Filtering the wells of the plate to dryness purifies the sequencing products by washing salts and unincorporated dye terminators to waste. The purified sequencing products are then resuspended from the membrane surface and are ready for injection into a capillary or slab gel type DNA Sequencer. |
