Vaccines Learning Center

Vaccines are biologically active products designed to stimulate the immune system against future invasion of a pathogen by inducing memory to specific antigens. Vaccines may be produced from viral pathogens such as those causing polio, influenza and Lyme Disease, or from bacterial pathogens such as pertussis, tetanus, diphtheria and anthrax. Recombinant fermentation techniques such as those used for hepatitis vaccine, produce highly purified antigen-specific subunits that stimulate immunity without concern of pathogen infection. Vaccine production typically uses several filtration and purification steps. To obtain a purified vaccine the selected microorganism must first be grown then the selected antigen must be separated, purified, formulated and sterilized prior to delivery.

MAMMALIAN CELL		VIRAL VECTORS
	Essential Process Requirements: Cell Harvesting, Clarification or Polishing (Post TFF or Centrifuge), Bioburden Reduction, Concentration and Diafiltration (Prior to column chromatography steps), Concentration and Diafiltration (For final formulation), Sterile Filtration... Learn More »		Essential Process Requirements: Virus Barrier, Clarification, Bioburden Reduction, Concentration and Diafiltration (Prior to column chromatography steps), Concentration and Diafiltration (For final formulation), Sterile Filtration... Learn More »
PLASMID DNA		CONJUGATES
	Essential Process Requirements: Clarification and Solids Removal (in place of bag filter), Concentration and Diafiltration (prior to column chromatography steps), Concentration and Diafiltration (for final formulation), Sterile Filtration... Learn More »		Essential Process Requirements: Concentration and Diafiltration (Prior to additional purification), Bioburden Reduction, Concentration and Diafiltration (Prior to column chromatography steps), Sterile Filtration... Learn More »
POLYSACCHARIDES		BIBLIOGRAPHY
	Essential Process Requirements: Primary Clarification, Secondary Clarification, Bioburden Reduction, Concentration and Diafiltration (For final formulation), Prefiltration (Bulk intermediate)... Learn More »		More information on vaccine related reports, case studies and product references... Learn More »

Application Requirements

• Vaccine broths have moderate to high solids concentrations that require effective removal before downstream purification. Millipore products are designed for high yield, throughput and efficiency.
• Highly purified vaccines are often required to minimize adverse reaction.
• Injectable vaccines require sterilization.

Vaccines	Batch Volumes
Mammalian Cell	<2,000 L
Viral Vectors	<500 L
Plasmid DNA	<5,000 L
Conjugates	<1,000 L
Polysaccharides	<2,000 L

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